5Z83
Crystal structure of GenB1 from Micromonospora echinospora in complex with PLP (internal aldimine)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-15 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.140, 88.012, 116.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.798 - 1.700 |
R-factor | 0.1591 |
Rwork | 0.158 |
R-free | 0.19230 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.190 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_2474) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.055 | 0.241 |
Rpim | 0.017 | 0.123 |
Number of reflections | 84747 | 3524 |
<I/σ(I)> | 34 | |
Completeness [%] | 97.2 | |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 295 | magnesium formate, PEG3350 |