5Z81

Trimeric structure of Vibrio cholerae Heat Shock Protein 15 at 2.3 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	293.15
Detector technology	AREA DETECTOR
Collection date	2014-07-23
Detector	MARRESEARCH
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	53.650, 90.280, 81.090
Unit cell angles	90.00, 103.92, 90.00

Refinement procedure

Resolution	39.354 - 2.334
R-factor	0.2309
Rwork	0.228
R-free	0.25940
RMSD bond length	0.012
RMSD bond angle	1.691
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.350	2.340
High resolution limit [Å]	2.330	2.330
Rmerge	0.010
Number of reflections	15374
<I/σ(I)>	5.9
Completeness [%]	93.0
Redundancy	2.02

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293.15	2.4M Ammonium Sulphate, 5% MPD.