5Z7Z
Crystal structure of Striga hermonthica Dwarf14 (ShD14)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2015-10-31 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.662, 70.316, 84.684 |
Unit cell angles | 90.00, 94.03, 90.00 |
Refinement procedure
Resolution | 38.313 - 1.978 |
R-factor | 0.2103 |
Rwork | 0.208 |
R-free | 0.25410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vxk |
RMSD bond length | 0.004 |
RMSD bond angle | 0.648 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 1.978 | 1.978 |
Rmeas | 0.065 | 0.582 |
Number of reflections | 36505 | 2707 |
<I/σ(I)> | 17.6 | 3 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.999 | 0.872 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 10 | 293.15 | 100mM CAPS (pH 10.0), 200mM NaCl, 20% (w/v) PEG 8000 |