5Z7W
Crystal structure of Striga hermonthica HTL1 (ShHTL1)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2017-02-13 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 81.170, 138.770, 49.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.953 - 1.657 |
R-factor | 0.1708 |
Rwork | 0.169 |
R-free | 0.19930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jyp |
RMSD bond length | 0.004 |
RMSD bond angle | 0.657 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.700 |
High resolution limit [Å] | 1.657 | 1.657 |
Rmeas | 0.071 | 0.875 |
Number of reflections | 127266 | 8897 |
<I/σ(I)> | 18 | 2.2 |
Completeness [%] | 99.4 | 93.7 |
Redundancy | 6.7 | 6.2 |
CC(1/2) | 0.999 | 0.818 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris-HCl (pH 8.5), 200mM MgCl2, 30% (w/v) PEG 4000 |