5Z7F
Horse Heart Myoglobin Mutant -V68A/I107Y-Sulfide Derivative
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-05-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97845 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.003, 28.516, 62.696 |
Unit cell angles | 90.00, 105.61, 90.00 |
Refinement procedure
Resolution | 60.380 - 1.900 |
R-factor | 0.16674 |
Rwork | 0.164 |
R-free | 0.22740 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.845 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.380 | 1.949 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 8914 | |
<I/σ(I)> | 15 | |
Completeness [%] | 97.0 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.5-3.0M ammonium sulfate, 0.1M Tris |