5Z7E
Horse Heart Myoglobin Mutant - H93M
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-24 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.082, 28.725, 63.271 |
| Unit cell angles | 90.00, 105.76, 90.00 |
Refinement procedure
| Resolution | 60.890 - 1.800 |
| R-factor | 0.15837 |
| Rwork | 0.157 |
| R-free | 0.19720 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.783 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.890 | 1.847 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 10965 | |
| <I/σ(I)> | 20 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2.5-3.0M ammonium sulfate, 0.1M Tris |
