5Z7C
crystal structure of cyclic GMP-AMP specifc phosphodiesterases in V.cholerae (V-cGAP3)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 139.360, 139.360, 179.210 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.595 - 2.760 |
| R-factor | 0.2475 |
| Rwork | 0.246 |
| R-free | 0.26750 |
| Structure solution method | SAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 1.682 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.760 | 2.760 |
| Rmerge | 0.081 | 0.898 |
| Number of reflections | 26853 | |
| <I/σ(I)> | 17.85 | |
| Completeness [%] | 99.2 | |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | 20% (w/v) PEG 3350, 0.2M ammonium citrate tribasic, pH 7.0 |
