5Z71
Crystal structure of the Homo Sapiens cytoplasmic ribosomal decoding site in complex with G418 (P21212 form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-10 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 30.924, 90.106, 46.701 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.053 - 2.500 |
R-factor | 0.2731 |
Rwork | 0.270 |
R-free | 0.30510 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.487 |
Data scaling software | XSCALE |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.053 | 45.053 | 2.560 |
High resolution limit [Å] | 2.500 | 11.170 | 2.500 |
Rmerge | 0.029 | 0.022 | 0.313 |
Rmeas | 0.034 | 0.026 | 0.364 |
Number of reflections | 8669 | 66 | 648 |
<I/σ(I)> | 25.53 | 46.26 | 3.61 |
Completeness [%] | 98.9 | 66.7 | 97.9 |
Redundancy | 3.827 | 3.424 | 3.73 |
CC(1/2) | 0.999 | 0.999 | 0.909 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | MPD, Sodium chloride, Sprimine 4HCl, Sodium cacodylate (pH 5.0) |