5Z6Y

Structure of sfYFP48S95C66BPA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	200
Detector technology	CCD
Collection date	2017-12-30
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	51.574, 51.574, 179.459
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.849 - 1.870
R-factor	0.21
Rwork	0.209
R-free	0.23580
RMSD bond length	0.008
RMSD bond angle	1.041
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.870	1.870
<I/σ(I)>	15.8
Completeness [%]	100.0
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	289	0.1M sodium malonate pH 4.0, 12% PEG 3350