5Z6V
Crystal structure of a substrate-binding protein from Rhodothermus marinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-09-27 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.900, 63.320, 34.666 |
| Unit cell angles | 90.00, 97.22, 90.00 |
Refinement procedure
| Resolution | 44.800 - 1.870 |
| R-factor | 0.15596 |
| Rwork | 0.154 |
| R-free | 0.19840 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.030 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.870 | 1.900 |
| Rmerge | 0.133 | 0.381 |
| Rmeas | 0.145 | 0.410 |
| Rpim | 0.057 | 0.150 |
| Number of reflections | 10741 | 530 |
| <I/σ(I)> | 48.38 | |
| Completeness [%] | 98.0 | 97.8 |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 295.5 | sodium acetate, MgCl2, PEG 3350 |
