5Z6O
Crystal structure of Penicillium cyclopium protease
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 290 |
Detector technology | AREA DETECTOR |
Collection date | 1995-04-01 |
Detector | UCSD MARK II |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.223, 61.642, 70.862 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.721 - 1.700 |
R-factor | 0.1441 |
Rwork | 0.142 |
R-free | 0.18090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2prk |
RMSD bond length | 0.011 |
RMSD bond angle | 1.073 |
Data reduction software | UCSD-system |
Data scaling software | Aimless |
Phasing software | X-PLOR |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.088 | 0.362 |
Number of reflections | 33985 | 1580 |
<I/σ(I)> | 10.6 | 2.8 |
Completeness [%] | 97.5 | 92.7 |
Redundancy | 5.3 | 3.3 |
CC(1/2) | 0.996 | 0.544 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 15% PEG 4000 |