5Z6D
Crystal structure of Abundant Perithecial Protein (APP) from Neurospora crassa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E+ SUPERBRIGHT |
Temperature [K] | 93 |
Detector technology | IMAGE PLATE |
Collection date | 2010-06-06 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 46.319, 40.168, 103.542 |
Unit cell angles | 90.00, 96.81, 90.00 |
Refinement procedure
Resolution | 23.031 - 1.600 |
R-factor | 0.1859 |
Rwork | 0.184 |
R-free | 0.22890 |
Structure solution method | MIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 1.039 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.053 | 0.245 |
Number of reflections | 45708 | 3831 |
<I/σ(I)> | 29.2 | 6 |
Completeness [%] | 91.1 | 77.4 |
Redundancy | 4.4 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20-25% v/v PEG MME 550, 10-30mM CaCl2, 100mM Bis-Tris, pH 5.5-6.5 |