5Z6B
Crystal structure of sugar-binding protein YesO in complex with rhamnogalacturonan trisaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-16 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.230, 78.592, 54.075 |
Unit cell angles | 90.00, 102.04, 90.00 |
Refinement procedure
Resolution | 43.877 - 1.582 |
R-factor | 0.1807 |
Rwork | 0.179 |
R-free | 0.20430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r6k |
RMSD bond length | 0.006 |
RMSD bond angle | 0.851 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.610 |
High resolution limit [Å] | 1.580 | 1.580 |
Number of reflections | 46602 | |
<I/σ(I)> | 30.4 | 4.5 |
Completeness [%] | 97.9 | 88.2 |
Redundancy | 2.5 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% (w/v) PEG 1000, 0.1M Tris-HCl (pH 8.5) , 25% glycerol |