5Z6B
Crystal structure of sugar-binding protein YesO in complex with rhamnogalacturonan trisaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-16 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.230, 78.592, 54.075 |
| Unit cell angles | 90.00, 102.04, 90.00 |
Refinement procedure
| Resolution | 43.877 - 1.582 |
| R-factor | 0.1807 |
| Rwork | 0.179 |
| R-free | 0.20430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r6k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.851 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Number of reflections | 46602 | |
| <I/σ(I)> | 30.4 | 4.5 |
| Completeness [%] | 97.9 | 88.2 |
| Redundancy | 2.5 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 20% (w/v) PEG 1000, 0.1M Tris-HCl (pH 8.5) , 25% glycerol |
