5Z69
Structure of the recombination mediator protein RecF-ATPrS in RecFOR pathway
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.005 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 166.727, 48.107, 116.855 |
Unit cell angles | 90.00, 100.06, 90.00 |
Refinement procedure
Resolution | 19.835 - 2.102 |
R-factor | 0.1893 |
Rwork | 0.188 |
R-free | 0.22070 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.267 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 913873 | |
<I/σ(I)> | 31.2 | |
Completeness [%] | 99.6 | |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, 300 mM NaCl, 100 mM Tris-Hcl, pH 7.0 |