5Z67
Structure of the recombination mediator protein RecF in RecFOR pathway
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.005 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 49.839, 95.780, 167.456 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.992 - 2.200 |
R-factor | 0.1792 |
Rwork | 0.175 |
R-free | 0.22310 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.262 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
<I/σ(I)> | 60.7 | |
Completeness [%] | 99.0 | |
Redundancy | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 9 | 293 | 28% (w/v) PEG 3350, 300 mM NaCl, 100 mM Tris-Hcl, pH 9.0 |