5Z65
Crystal structure of porcine aminopeptidase N ectodomain in functional form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-20 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 260.327, 62.310, 80.569 |
| Unit cell angles | 90.00, 100.06, 90.00 |
Refinement procedure
| Resolution | 73.440 - 2.650 |
| R-factor | 0.19832 |
| Rwork | 0.196 |
| R-free | 0.25141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hom |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.694 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 73.440 | 2.700 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 37252 | |
| <I/σ(I)> | 7.7 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 298 | 100 mM Hepes pH 7.2, 0.2 M sodium fluoride, 25% PEG 3350 |
