5Z5P
Crystal structure of ConA-R3M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9716 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 58.742, 61.945, 115.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.740 - 1.400 |
R-factor | 0.19492 |
Rwork | 0.193 |
R-free | 0.24149 |
RMSD bond length | 0.025 |
RMSD bond angle | 2.647 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Number of reflections | 84005 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 99.7 | |
Redundancy | 12.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 300 | 20% polyethylene glycol 6000, 20 mM HEPES, 100 mM NaCl, 5 mM CaCl2, 5 mM MnCl2 pH 7.2 |