5Z5F
Crystal structure of a thermostable glycoside hydrolase family 43 {beta}-1,4-xylosidase from Geobacillus thermoleovorans IT-08 in complex with L-arabinose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-07-27 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9330 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 59.989, 59.989, 278.987 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.480 - 2.100 |
R-factor | 0.1519 |
Rwork | 0.149 |
R-free | 0.19950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yif |
RMSD bond length | 0.012 |
RMSD bond angle | 1.538 |
Data reduction software | COMBAT |
Data scaling software | SCALA (3.3.16) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.481 | 46.498 | 2.210 |
High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
Rmerge | 0.049 | 0.408 | |
Rmeas | 0.132 | 0.054 | 0.451 |
Rpim | 0.049 | 0.022 | 0.185 |
Number of reflections | 31118 | 1172 | 4397 |
<I/σ(I)> | 12 | 4.5 | 1.9 |
Completeness [%] | 99.9 | 99.7 | 99.5 |
Redundancy | 6.9 | 6.5 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 283 | 0.1M HEPES (N-(2-hydroxyethyl)piperazine-N-(2-ethanesulfonic acid)) buffer, pH 7.0, 5% (w/v) PEG 6000 |