5Z5C

Crystal structure of hydrogen sulfide-producing enzyme (Fn1055) from Fusobacterium nucleatum: lysine-dimethylated form

Replaces:  5B54

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2011-06-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	57.760, 136.160, 171.680
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.930 - 2.070
R-factor	0.18839
Rwork	0.186
R-free	0.24177
RMSD bond length	0.014
RMSD bond angle	1.597
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.660	2.120
High resolution limit [Å]	2.070	2.070
Rmerge	0.051	0.245
Number of reflections	83053
<I/σ(I)>	22.3	7.2
Completeness [%]	99.6	98.4
Redundancy	7	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	24% (W/V) PEG 3350, 0.1M HEPES PH 7.5, 0.2M MAGNESIUM CHLORIDE HEXAHYDRATE