5Z51
Helicase binding domain of primase from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 177 |
Detector technology | CCD |
Collection date | 2016-02-23 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 48.282, 137.559, 36.514 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.492 - 1.583 |
R-factor | 0.1827 |
Rwork | 0.181 |
R-free | 0.20880 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.641 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXCD |
Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.780 | 1.520 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.076 | |
Rmeas | 0.088 | |
Rpim | 0.044 | 0.299 |
Number of reflections | 40035 | |
<I/σ(I)> | 19.667 | |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 3.8 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 289 | PEG 3350, magnesium acetate, mops |