5Z4W
Crystal structure of signalling protein from buffalo (SPB-40) with an altered conformation of Trp78 at 1.79 A resolution
Replaces: 5WSMExperimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.068, 66.735, 106.072 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.490 - 1.790 |
R-factor | 0.152 |
Rwork | 0.150 |
R-free | 0.18600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4q7n |
RMSD bond length | 0.030 |
RMSD bond angle | 2.529 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.500 | 1.840 |
High resolution limit [Å] | 1.790 | 1.790 |
Number of reflections | 40939 | |
<I/σ(I)> | 2.1 | |
Completeness [%] | 99.6 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 35mM Tris-HCl, 25mM NaCl, 20% ethanol pH 8.0 |