5Z4V
Crystal structure of the sheep signalling glycoprotein (SPS-40) complex with 2-methyl-2-4-pentanediol at 1.65A resolution reveals specific binding characteristics of SPS-40
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2007-03-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.81 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.873, 66.415, 105.692 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.711 - 1.650 |
R-factor | 0.1385 |
Rwork | 0.138 |
R-free | 0.18760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pi6 |
RMSD bond length | 0.035 |
RMSD bond angle | 2.892 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.900 | 1.680 |
High resolution limit [Å] | 1.650 | 1.650 |
Number of reflections | 52135 | |
<I/σ(I)> | 13.3 | |
Completeness [%] | 99.9 | 99.7 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 298 | Tri-HCl, 20% Ethanol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |