5Z4D

Structure of Tailor in complex with AGUU RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-13
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9778
Spacegroup name	C 2 2 21
Unit cell lengths	61.571, 84.787, 148.118
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.820 - 1.803
R-factor	0.1589
Rwork	0.158
R-free	0.18430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nkt
RMSD bond length	0.010
RMSD bond angle	1.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.080	0.049	0.465
Rmeas	0.083	0.051	0.487
Rpim	0.023	0.014	0.141
Number of reflections	35714	1958	1670
<I/σ(I)>	5.1
Completeness [%]	98.9	100	95.3
Redundancy	12.5	12.1	11.1
CC(1/2)		0.999	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M Lithium chloride, 0.1M Tris pH 8.4, 15% w/v PEG 3350