5Z4A
Structure of Tailor in complex with AGU RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9778 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.717, 84.944, 148.262 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.930 - 1.637 |
R-factor | 0.1587 |
Rwork | 0.157 |
R-free | 0.19050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nkt |
RMSD bond length | 0.009 |
RMSD bond angle | 0.979 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.890 |
High resolution limit [Å] | 1.637 | 5.050 | 1.860 |
Rmerge | 0.086 | 0.055 | 0.493 |
Rmeas | 0.089 | 0.057 | 0.515 |
Rpim | 0.025 | 0.016 | 0.149 |
Number of reflections | 48194 | 1771 | 1442 |
<I/σ(I)> | 4.6 | ||
Completeness [%] | 99.5 | 100 | 93.1 |
Redundancy | 12.8 | 12.3 | 10.8 |
CC(1/2) | 0.999 | 0.948 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M Lithium chloride, 0.1M Tris pH 8.4, 15% w/v PEG 3350 |