5Z43

Crystal structure of prenyltransferase AmbP1 apo structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-09
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1000
Spacegroup name	P 41
Unit cell lengths	116.263, 116.263, 48.723
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.105 - 2.361
R-factor	0.1939
Rwork	0.190
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yk9
RMSD bond length	0.009
RMSD bond angle	0.973
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.900	2.450
High resolution limit [Å]	2.360	2.360
Rmerge	0.038	0.493
Number of reflections	27028	2823
<I/σ(I)>	18.8	2.3
Completeness [%]	99.6	99.2
Redundancy	3.9	3.9
CC(1/2)	0.999	0.807

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100mM MES (pH6.5), 0.2M MgCl2, 22% PEG8000