5Z3F

Glycosidase E335A in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 4 21 2
Unit cell lengths	104.830, 104.830, 89.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.880 - 1.100
R-factor	0.10827
Rwork	0.108
R-free	0.12089
RMSD bond length	0.018
RMSD bond angle	1.695
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.880	1.160
High resolution limit [Å]	1.100	1.100
Number of reflections	200183
<I/σ(I)>	16.39
Completeness [%]	99.9
Redundancy	18.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2M Ammonium citrate tribasic (pH 6.8), 0.1M Sodium citrate (pH 5.5), 12% PEGmonomethyl ether 2000, 2mM isomaltose