5Z3C
Glycosidase E178A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-04 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 104.917, 104.917, 89.499 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.257 - 1.600 |
| R-factor | 0.135 |
| Rwork | 0.134 |
| R-free | 0.15710 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.425 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.260 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 66196 | |
| <I/σ(I)> | 38.12 | |
| Completeness [%] | 99.9 | |
| Redundancy | 21.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M Ammonium citrate tribasic (pH 6.8), 0.1M Sodium citrate (pH 5.5), 12% PEGmonomethyl ether 2000, 2mM isomaltose |
