5Z39
Crystal structure of C terminal region of G-protein interacting protein 1 (Gip1) from Dictyostelium discoideum form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.700 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.468, 43.618, 101.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.085 - 2.740 |
| R-factor | 0.2265 |
| Rwork | 0.221 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5z1n |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.454 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.620 | 43.620 | 2.870 |
| High resolution limit [Å] | 2.740 | 9.090 | 2.740 |
| Rmerge | 0.122 | 0.044 | 0.611 |
| Rmeas | 0.127 | 0.046 | 0.634 |
| Rpim | 0.034 | 0.013 | 0.169 |
| Total number of observations | 57795 | ||
| Number of reflections | 4277 | 150 | 554 |
| <I/σ(I)> | 18.9 | ||
| Completeness [%] | 100.0 | 99 | 100 |
| Redundancy | 13.5 | 10.4 | 13.9 |
| CC(1/2) | 0.999 | 0.999 | 0.936 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 14-20% PEG 20000, 100 mM Bicine, |
