5Z38

Crystal structure of CsrA bound to CesT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-30
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97775
Spacegroup name	C 1 2 1
Unit cell lengths	252.750, 52.546, 93.356
Unit cell angles	90.00, 96.73, 90.00

Refinement procedure

Resolution	49.351 - 2.292
R-factor	0.234
Rwork	0.233
R-free	0.25130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k3e 2bti
RMSD bond length	0.003
RMSD bond angle	0.579
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.072
Rmeas	0.078
Rpim	0.030	0.557
Number of reflections	54958	5428
<I/σ(I)>	23.01	1.253
Completeness [%]	99.8	99.8
Redundancy	6.7	6.5
CC(1/2)	0.997	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	291	20% PEG 3350, 0.2M sodium citrate tribasic dihydrate