5Z38
Crystal structure of CsrA bound to CesT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-30 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97775 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 252.750, 52.546, 93.356 |
Unit cell angles | 90.00, 96.73, 90.00 |
Refinement procedure
Resolution | 49.351 - 2.292 |
R-factor | 0.234 |
Rwork | 0.233 |
R-free | 0.25130 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1k3e 2bti |
RMSD bond length | 0.003 |
RMSD bond angle | 0.579 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.072 | |
Rmeas | 0.078 | |
Rpim | 0.030 | 0.557 |
Number of reflections | 54958 | 5428 |
<I/σ(I)> | 23.01 | 1.253 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 6.7 | 6.5 |
CC(1/2) | 0.997 | 0.877 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 291 | 20% PEG 3350, 0.2M sodium citrate tribasic dihydrate |