5Z2V

Crystal structure of RecR from Pseudomonas aeruginosa PAO1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97776
Spacegroup name	P 61 2 2
Unit cell lengths	70.075, 70.075, 369.022
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.18
Rwork	0.178
R-free	0.21300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vdp
RMSD bond length	0.011
RMSD bond angle	1.584
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Rpim	0.034	0.215
Number of reflections	27394	2318
<I/σ(I)>	20.3	3.3
Completeness [%]	99.7	97.9
Redundancy	20	15
CC(1/2)	0.999	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.26M sodium phosphate mono basic monohydrate, 0.14M potassium phosphate dibasic, 10% PEG 6000, 100mM HEPES/sodium hydroxide pH 7.0