5Z2N
Structure of Orp1L N-terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.193, 30.061, 95.055 |
| Unit cell angles | 90.00, 126.06, 90.00 |
Refinement procedure
| Resolution | 46.276 - 2.140 |
| R-factor | 0.2385 |
| Rwork | 0.238 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k5b |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.743 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.300 | 2.270 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Number of reflections | 28304 | |
| <I/σ(I)> | 18.57 | 2.23 |
| Completeness [%] | 93.5 | |
| Redundancy | 3.4 | |
| CC(1/2) | 1.000 | 0.943 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 0.2 M NaCl, 0.1 M BIS-TRIS pH 6.5, 25% PEG 3350 |
