5Z2I
Structure of Dictyostelium discoideum mitochondrial calcium uniporter N-ternimal domain (Se-DdMCU-NTD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97776 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.455, 70.832, 60.005 |
Unit cell angles | 90.00, 92.17, 90.00 |
Refinement procedure
Resolution | 42.425 - 2.141 |
R-factor | 0.1927 |
Rwork | 0.191 |
R-free | 0.23420 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.740 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.770 | 2.200 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.099 | 0.823 |
Rmeas | 0.105 | 0.881 |
Rpim | 0.034 | 0.305 |
Number of reflections | 19374 | 1416 |
<I/σ(I)> | 13.9 | 2.8 |
Completeness [%] | 98.7 | 88.6 |
Redundancy | 9.4 | 8 |
CC(1/2) | 0.998 | 0.723 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 20%(v/v) PEG4000, 10%(v/v) 2-propanol, 0.01M HEPES (pH 7.5) |