5Z2G
Crystal Structure of L-amino acid oxidase from venom of Naja atra
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-05-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 104.836, 104.836, 214.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.433 - 2.676 |
| R-factor | 0.1738 |
| Rwork | 0.171 |
| R-free | 0.21960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iid |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.247 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.730 |
| High resolution limit [Å] | 2.670 | 2.680 |
| Rmerge | 0.068 | 0.516 |
| Number of reflections | 39179 | 1911 |
| <I/σ(I)> | 2.5 | |
| Completeness [%] | 99.5 | |
| Redundancy | 10.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293.15 | 0.1mM Hepes, 10% PEG 6000 |
