5Z22
Crystal Structure of Laccase from Cerrena sp. RSD1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-03 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 87.409, 87.409, 148.791 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.500 |
R-factor | 0.12699 |
Rwork | 0.126 |
R-free | 0.15195 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z1x |
RMSD bond length | 0.007 |
RMSD bond angle | 1.338 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.064 | 0.630 |
Number of reflections | 89774 | 8972 |
<I/σ(I)> | 27.5 | 1.9 |
Completeness [%] | 96.8 | 98.1 |
Redundancy | 4.7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.4 | 293 | 1.6M almmonium sulfate, pH8.4 |