5Z1Q
Crystal structures of the trimeric N-terminal domain of Ciliate Euplotes octocarinatus Centrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-01-14 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9779 |
Spacegroup name | P 1 |
Unit cell lengths | 42.945, 43.045, 43.087 |
Unit cell angles | 106.88, 106.89, 106.61 |
Refinement procedure
Resolution | 37.653 - 2.300 |
R-factor | 0.1859 |
Rwork | 0.184 |
R-free | 0.22430 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.386 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.10.1) |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmeas | 0.246 | 0.312 |
Number of reflections | 9561 | |
<I/σ(I)> | 5.27 | |
Completeness [%] | 97.5 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 100mM NaH2PO4 (pH=4.2) and 40%(w/v) PEG600 |