5Z1N
Crystal structure of C terminal region of G-protein interacting protein 1 (Gip1) from Dictyostelium discoideum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-20 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.318, 44.143, 96.637 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.319 - 1.949 |
R-factor | 0.1847 |
Rwork | 0.182 |
R-free | 0.23040 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3f4m |
RMSD bond length | 0.003 |
RMSD bond angle | 0.602 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
High resolution limit [Å] | 1.949 | 5.290 | 1.950 |
Rmerge | 0.090 | 0.045 | 0.353 |
Rmeas | 0.105 | 0.054 | 0.424 |
Rpim | 0.054 | 0.030 | 0.232 |
Number of reflections | 10906 | 645 | 359 |
<I/σ(I)> | 8.2 | ||
Completeness [%] | 98.3 | 99.5 | 66.2 |
Redundancy | 3.8 | 3.3 | 3.2 |
CC(1/2) | 0.996 | 0.832 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 14-20% PEG 20000, 100mM Bicine, |