5Z1H
Crystal structure of the bacterial ribosomal decoding site in complex with 6'-fluoro sisomicin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-14 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.905, 31.665, 46.696 |
Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
Resolution | 42.800 - 2.400 |
R-factor | 0.2171 |
Rwork | 0.212 |
R-free | 0.26320 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.008 |
Data scaling software | d*TREK (9.4SSI) |
Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.800 | 42.750 | 2.490 |
High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
Rmerge | 0.084 | 0.057 | 0.272 |
Rmeas | 0.084 | ||
Number of reflections | 5052 | 523 | 486 |
<I/σ(I)> | 8.5 | 16.5 | 3.2 |
Completeness [%] | 99.7 | 98.1 | 100 |
Redundancy | 3.54 | 3.29 | 3.61 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 2-Methyl-2,4-pentanediol, LiCl, spermine tetrahydrochloride, sodium cacodylate |