5Z1G
Structure of the Brx1 and Ebp2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.934, 47.353, 100.695 |
| Unit cell angles | 90.00, 94.10, 90.00 |
Refinement procedure
| Resolution | 24.909 - 2.294 |
| R-factor | 0.2331 |
| Rwork | 0.230 |
| R-free | 0.29740 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.294 | 2.294 |
| Rmerge | 0.099 | 0.382 |
| Number of reflections | 33644 | 1715 |
| <I/σ(I)> | 17.8 | 4 |
| Completeness [%] | 97.5 | 99.9 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M Bis-Tris, pH 5.5 and 1.8M ammonium sulfate |
