5Z15
Crystal structure of human TLR8 in complex with CU-CPT9c
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2016-10-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.892, 98.747, 139.476 |
Unit cell angles | 90.00, 109.04, 90.00 |
Refinement procedure
Resolution | 131.850 - 2.900 |
R-factor | 0.2059 |
Rwork | 0.202 |
R-free | 0.28922 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.591 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 131.850 | 2.950 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 41014 | |
<I/σ(I)> | 17.8 | |
Completeness [%] | 98.8 | |
Redundancy | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 4000, calcium chloride, Tris-HCl, ethylene glycol |