5Z14
Crystal structure of human TLR8 in complex with CU-CPT9a
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2016-11-18 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0000 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 143.510, 99.000, 139.490 |
Unit cell angles | 90.00, 108.51, 90.00 |
Refinement procedure
Resolution | 44.090 - 2.800 |
R-factor | 0.20456 |
Rwork | 0.201 |
R-free | 0.27698 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.567 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 132.270 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 43515 | |
<I/σ(I)> | 14.2 | |
Completeness [%] | 99.0 | |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 4000, calcium chloride, Tris-HCl, ethylene glycol |