5Z0U
Thermoactinomyces vulgaris R-47 alpha-amylase I (TVA I) 11 residues (from A363 to N373) deletion mutant (Del11)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.972, 54.022, 77.907 |
| Unit cell angles | 90.00, 100.07, 90.00 |
Refinement procedure
| Resolution | 49.740 - 1.370 |
| R-factor | 0.13831 |
| Rwork | 0.138 |
| R-free | 0.15020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ji1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.352 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.440 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.048 | 0.168 |
| Number of reflections | 121625 | 16025 |
| <I/σ(I)> | 14.1 | 5.1 |
| Completeness [%] | 92.3 | 83.8 |
| Redundancy | 2.7 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 20% MPD, 20% PEG6000, 40 mM MES-NaOH |
