5Z0Q
Crystal Structure of OvoB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97853 |
Spacegroup name | P 1 |
Unit cell lengths | 58.123, 133.590, 165.703 |
Unit cell angles | 89.39, 84.58, 86.56 |
Refinement procedure
Resolution | 42.973 - 2.770 |
R-factor | 0.2125 |
Rwork | 0.210 |
R-free | 0.26690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4dgt |
RMSD bond length | 0.002 |
RMSD bond angle | 0.513 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.820 |
High resolution limit [Å] | 2.770 | 2.770 |
Number of reflections | 125870 | |
<I/σ(I)> | 7.05 | |
Completeness [%] | 96.7 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | PEG 8000, sodium cacodylate, calcium acetate, glycerol |