5Z0A
ST0452(Y97N)-GlcNAc binding form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.843, 94.951, 101.906 |
| Unit cell angles | 110.62, 101.47, 113.03 |
Refinement procedure
| Resolution | 87.790 - 2.090 |
| R-factor | 0.19481 |
| Rwork | 0.193 |
| R-free | 0.23154 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ggo |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.859 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 87.800 | 2.150 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.072 | 0.415 |
| Number of reflections | 154041 | 7531 |
| <I/σ(I)> | 22 | |
| Completeness [%] | 96.9 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% (w/v) PEG3350, 0.2M potassium citrate tribasic monohydrate |
