5Z08
The crystal structure of kinetochore subunits Cenp-H/I/K triple complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-15 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.986 |
Spacegroup name | P 41 |
Unit cell lengths | 67.847, 67.847, 186.269 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.975 - 2.199 |
R-factor | 0.1898 |
Rwork | 0.188 |
R-free | 0.22980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.901 |
Data reduction software | PHASER |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2463)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.199 | 2.200 |
Number of reflections | 42600 | |
<I/σ(I)> | 29.6 | |
Completeness [%] | 100.0 | |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 8000 |