5Z05
Crystal structure of signalling protein from buffalo (SPB-40) with an acetone induced conformation of Trp78 at 1.49 A resolution
Replaces: 5WSIReplaces: 4S06Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-10-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.457, 66.517, 105.847 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.320 - 1.490 |
R-factor | 0.14856 |
Rwork | 0.147 |
R-free | 0.17641 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5WSI |
RMSD bond length | 0.038 |
RMSD bond angle | 3.117 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.320 | 1.530 |
High resolution limit [Å] | 1.490 | 1.490 |
Number of reflections | 65450 | |
<I/σ(I)> | 11.4 | 2.2 |
Completeness [%] | 99.0 | 99 |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | 35mM Tris -HCl pH- 8.0, 25mM Nacl, 20% Acetone. |