5Z00

AtVAL1 B3 domain in complex with 15bp-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	68.223, 97.416, 71.149
Unit cell angles	90.00, 110.19, 90.00

Refinement procedure

Resolution	29.202 - 2.587
R-factor	0.2186
Rwork	0.216
R-free	0.26760
RMSD bond length	0.013
RMSD bond angle	1.539
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.780	2.690
High resolution limit [Å]	2.587	2.600
Rmerge	0.150
Number of reflections	27090
<I/σ(I)>	12.2
Completeness [%]	77.0
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M Bicine pH 8.5, 30% (w/v) PEG 6000