5YZY

AtVAL1 B3 domain in complex with 13bp-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97853
Spacegroup name	I 41 2 2
Unit cell lengths	87.780, 87.780, 148.735
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.610
R-factor	0.21238
Rwork	0.210
R-free	0.25674
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.695
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	SHELXDE
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.600	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.096
Number of reflections	9243
<I/σ(I)>	37.3
Completeness [%]	100.0
Redundancy	16.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% (w/v) PEG 5000 MME, 0.1M Tris base/Hydrochloric acid, 0.2M Lithium sulfate