5YZW
Crystal structure of p204 HINb domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-03-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 99.720, 99.720, 107.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.607 - 2.001 |
| R-factor | 0.2069 |
| Rwork | 0.204 |
| R-free | 0.25440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rlo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.844 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.074 | 1.366 |
| Number of reflections | 41767 | 6643 |
| <I/σ(I)> | 18.35 | |
| Completeness [%] | 99.6 | 99.4 |
| Redundancy | 10.7 | 10.85 |
| CC(1/2) | 0.999 | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 20% PEG300, 100 mM Bicine pH 8.5 |
