5YZW
Crystal structure of p204 HINb domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2012-03-09 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 99.720, 99.720, 107.050 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.607 - 2.001 |
R-factor | 0.2069 |
Rwork | 0.204 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rlo |
RMSD bond length | 0.007 |
RMSD bond angle | 0.844 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmeas | 0.074 | 1.366 |
Number of reflections | 41767 | 6643 |
<I/σ(I)> | 18.35 | |
Completeness [%] | 99.6 | 99.4 |
Redundancy | 10.7 | 10.85 |
CC(1/2) | 0.999 | 0.841 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 20% PEG300, 100 mM Bicine pH 8.5 |