5YZP
Crystal structure of p204 HINa domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-02 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.930, 104.210, 90.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.178 - 1.581 |
| R-factor | 0.1849 |
| Rwork | 0.184 |
| R-free | 0.21050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oq0 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.843 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2481: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmeas | 0.058 | 0.710 |
| Number of reflections | 37551 | 5887 |
| <I/σ(I)> | 20.45 | |
| Completeness [%] | 99.3 | 97.7 |
| Redundancy | 7.66 | 7 |
| CC(1/2) | 0.998 | 0.899 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 23% PEG4000, 0.2M Ammonium Acetate, 100 mM NaAcO pH 5.0 |
