5YZL
Crystal structure of human Archease D178A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-11-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0688 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.686, 59.620, 133.192 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.730 - 3.400 |
Rwork | 0.188 |
R-free | 0.25120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yz1 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MLPHARE |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.460 |
High resolution limit [Å] | 3.400 | 3.400 |
Number of reflections | 5767 | |
<I/σ(I)> | 2 | |
Completeness [%] | 98.6 | |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290 | 30% PEG 400, 100mM HEPES/Sodium hydroxide pH 7.5, 200mM Magnesium chloride |